Silica microspheres in the anhydrous and aqueous media are prone to self-assembly, which would seriously affect the properties of the particle research. In order to study the use of silica microspheres and particles, the research on the de-aggregation of silica microspheres needs to be carried out. We propose a method which is able to achieve the reunion of silica microspheres and particles. We experimentally study the characteristics of different scales reunion particles and phenomena. By using a Nikon microscope, it shows that silica microspheres de-aggregation can be well done, and the dispersion and stability of the particles can be improved.